Endocrine Abstracts

Nodular thyroid disease is common and its pathogenesis remains poorly understood. Previously, we showed that thyroid-targeted overexpression of the proto-oncogene PTTG-Binding Factor (PBF) induced striking thyroid gland enlargement in transgenic mice (PBF-Tg)1. We have now investigated whether dietary factors influence goitrogenesis and the development of hyperplastic lesions in PBF-Tg mice. The mean weight of thyroids (5.8±0.9 mg; n=70) from 7 week old ...

The human pituitary tumor transforming gene (hPTTG) is overexpressed in thyroid cancers; it induces genetic instability and propagates growth through the induction of growth factors. We investigated the pathways of interaction between hPTTG and epidermal growth factor (EGF), transforming growth factor-α (TGF-α), insulin-like growth factor 1 (IGF1) and basic fibroblast growth factor (FGF2) in vitro and in vivo. Synchronised K1 and TPC-1 papillary thyroid...

PTTG binding factor (PBF) is a poorly characterised transforming gene that is overexpressed in thyroid tumours and inhibits the activity of the sodium iodide symporter (NIS) in vitro. Our recent investigations demonstrated that PBF mRNA expression was 2.6-fold higher in thyroid tissue excised from patients with multinodular goitres (MNG) than in normal thyroid tissue (n=25, P<0.01). We have now generated a murine transgenic model of targeted overexpres...

The human pituitary tumor transforming gene (hPTTG) is overexpressed in thyroid cancers; it induces genetic instability and propagates growth through the induction of growth factors. We set out to investigate the autocrine and paracrine pathways of interaction between hPTTG and epidermal growth factor (EGF), transforming growth factor-α (TGF-α) and insulin-like growth factor 1 (IGF1) in vitro and in vivo. Synchronised K1 papillary thyroid carcinoma cell...

Previously we showed that thyroid-targeted overexpression of the PTTG binding factor (PBF) induced significant goitrogenesis in transgenic mice (PBF-Tg). In 10 week old PBF-Tg mice thyroid weight was increased by 1.8±0.3-fold compared to wild-type (WT) controls (n=20, P<0.0001). We have now examined the long-term effects of PBF overexpression on thyroid gland weight and histology in aged PBF-Tg mice. Our study shows that the penetrance of increased thyro...